Cover Legend: Morphological changes over time in SLO-induced endosomes generated during plasma membrane repair.

NRK fibroblasts were incubated with the pore forming toxin SLO (upper panels) or not (lower panels) in the presence of BSA-gold for 5, 15 or 30 min and processed for transmission EM. Shortly after cell permeabilization SLO is endocytosed, ubiquitinated and subsequently sorted by the ESCRT complex into intraluminal vesicles of MVBs, where it is degraded. SLO-induced endosomes are more abundant, larger and enter late endocytic compartments faster than the constitutive endosomes observed in control cells. See Corrotte et al. Traffic 2012; 13(3): 483-494.
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Cover Legend: Morphology of a virus-containing intracellular plasma membrane-connected compartment (IPMC) in an HIV-1 infected human macrophage.

Human monocyte-derived macrophages infected with HIV-1 for 14 days were fixed and embedded in Epon for transmission EM. HIV particles are seen in an IPMC near the cell surface. The membranes connecting the IPMC to the cell surface are decorated on their cytoplasmic surface by ˜30 nm thick electron-dense coats, thicker and more uniform than the radially stippled clathrin-coated pits nearby. Immunolabelling studies demonstrate that these coats contain the ß2 integrin CD18 and the cytoskeletal linker proteins talin, vinculin and paxillin that connect the clustered integrins to the actin cytoskeleton. These coated structures maintain the integrity of HIV-1 assembly compartments in primary human macrophages. See Pelchen-Matthews et al. (Traffic 2012; 13(2): 273-291).
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Cover Legend: Subcellular localization of the potassium channel Kv7.1 during a calcium switch in MDCK cells.

Illustrated are 3D representations of confocal z-stacks of Kv7.1-expressing MDCK cells 0 hours, 30 minutes, 3 hours and 24 hours after initiation of the calcium switch. Kv7.1 is shown in green, a marker of the endoplasmic reticulum in red and the nucleus in blue. As illustrated, Kv7.1 has a very dynamic subcellular localization pattern during the switch (see Andersen et al. Traffic 2012; 13(1): 143-156).
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