Here are the cover illustrations of volume 3 issues. Please click the appropriate link to view the illustrations of other volumes:

• volume 13
• volume 12
• volume 11
• volume 10
• volume 9
• volume 8
• volume 7
• volume 6
• volume 5
• volume 4
• volume 2
• volume 1

Vol.3 Iss.12, Dec 2002	Cover Illustration: C5a eceptor structure. The image shows a model of the transmembrane, helical domains of the C5a receptor based on the crystal structure of rhodopsin as seen from the extracellular side.Helices are gray,and the colored residues (I124 purple, L127 red, F251 yellow,and N296 cyan)represent mutations that affect the activation and endocytic properties of the C5a receptor (see related article page 866).A four mutated residues are buried deeply within and oriented inside the transmembrane pocket that is formed by the seven helices, far from the receptors cytoplasmic loops.Hence,while the cytoplasmic loops contact the trimeric G protein and the endocytic machinery the mutated residues do not,suggesting the mutations affect conformational change indirectly and at a distance. Click for enlargement
Vol.3 Iss.11, Nov 2002	Cover Illustration: MxA GTPase interferes with intracellular transport of essential viral components. MxA (green) blocks the transport of LaCrosse virus nucleocapsid protein (red) to the Golgi compartment by sequestering the viral protein into large perinuclear complexes (yellow). An uninfected (middle)and 4 infected cells (bottom) are shown. Haller and Kochs. Traf ?c 2002;3(10):708 –715.(Photo by Mike Reichelt). Click for enlargement
Vol.3 Iss.10, Oct 2002	Cover Illustration: MxA GTPase interferes with intracellular transport of essential viral components. MxA (green)blocks the transport of LaCrosse virus nucleocapsid protein (red)to the Golgi compartment by sequestering the viral protein into large perinuclear complexes (yellow). An uninfected (middle)and 4 infected cells (bottom) are shown.Haller and Kochs.Traf ?c 2002;3(10):708 –715. (Photo by Mike Reichelt). Click for enlargement
Vol.3 Iss.9, Sept 2002	Cover Illustration: Distribution and colocalization of Ran-binding protein 2 (RanBP2) and kinesin heavy chains,KIF5C/KIF5A,in the bovine etinae. Radial cryosection (3 um)and overlay image of a retinal section double-immunostained with anti-RanBP2 (green)and H1 mAb against KIF5C/KIF5A (red). Nuclei are stained in b ue by DAPI.The H1 antibody immunostained strongly the myoid compartment of all photoreceptors and the nerve fiber layer (axons of ganglion cells).KIF5C/KIF5A was also localized at the distal tip of the ellipsoid compartment,consistent with its localization at the connecting cilium.In a rare subclass of blue cones (middle), KIF5C/KIF5A expression is absent from the myoid compartment and RanBP2 distribution is uninterrupted throughout the inner segment. RanBP2 is localized throughout the ellipsoid compartment of all photoreceptors and at the nuclear envelope of most retinal neurons. Its expression is extremely high in ganglion cells with a arge soma.Among photoreceptors, RanBP2 predominates at the nuclear pores of the nuclear envelope of cone nuclei. KIF5C/KIF5A interfaces with RanBP2 at the NE and boundary region between myoid and ellipsoid compartments of bovine photoreceptors. Click for enlargement
Vol.3 Iss.8, Aug 2002	Cover Illustration: Interactions between Hsc70 and auxilin are essential for vesicle uncoating in living nerve terminals. Colorized electron micrograph of an active zone from a nerveterminal in which uncoating was inhibited by microinjection of a mutant form of auxilin in which the HPD motif was mutated to AAA (Auxilin D HPD). Coated vesicles are colored purple.Auxilin D HPD is recruited to clathrin coated vesicles via interactions with its wild type clathrin binding domain,but is then unable to recruit and activate Hsc70 due to the mutation intheHsc70bindingsite. Statistica analyses of a large number of active zones revealed that there was a 5 fold increase in the number of coated vesicles in the terminals injected with auxilin D HPD vs wild type auxilin. Image courtesy of Eileen M.Lafer,George J.Augustine, JenniferMorgan, and Kondury Prasad.(see Morgan J R , Prasad K, Jin S, Augustine G.Jand Lafer EM. Uncoating of clathrin-coated vesicles in presynaptic terminals:roles for Hsc70 and auxilin.Neuron2001;32:289 –300). Click for enlargement
Vol.3 Iss.7, July 2002	Cover Illustration: In each ommatidium, the single unit of a Drosophila eye,there are eight photoreceptor cells and each contains the specialized organelle that houses the phototrans-duction machinery known as the rhabdomere. Pictured here are seven of the eight developing rhabdomeres (the eighth is out of the plane of focus)labeled by antibodies against Am-phiphysin and Actin at 72 hours after puparium formation. Each rhabdomere develops on the apical surface of the photoreceptor cell and extendt towards the inter-rhabdomeral space.Actin marks the microvilli core of the rhabdomeres,whereas Amphiphysin localizes to the outward edge of the growing microvilli.(see Review by Zhang and Zelhof,on page 452–460 in this issue). Click for enlargement
Vol.3 Iss.6, June 2002	Cover Illustration: Cortical vesicle exocytosis and compensatory endocytosis imaged by confocal microscopy. Living sea urchin eggs were attached to a polylysine treated glass coverslip and placed into a microscope perfusion chamber. Eggs were perfused into seawater containing the ?uid phase marker tetra-methylrhodamine dextran (3,000 mol.wt., RED ?uorescence) and the membrane marker FM1-43 (YELLOW ?uorescence).An optical section approximately 20 m above the glass surface through portions of two unfertilized eggs is shown in the left panel. The same eggs were imaged immediately after perfusion with sperm, but before fertilization envelope elevation,and is shown in the right panel. Note that the bottom egg has been fertilized, and now there are large (1 –2 m diameter) subcortical vesicles labeled by our probes.(Boris Baibakov and Steven S.Vogel,Traffic 2002;3:397–406) Click for enlargement
Vol.3 Iss.5, May 2002	Cover Illustration: Caveosomes: Intermediate organelles involved in caveolae-and lipid raftmediated endocytosis. Green African Monkey CV-1 cells that transiently express caveolin-1-GFP (green)were incubated with A exa Fluor 594 abeled Simian Virus 40 particles (red)for 3 hours at 37C and observed under a confocal uorescence microscope.The caveolin-1 containing caveosomes have accumulated virus particles.From caveosomes,SV40 is sorted to the ER and certain glcyolipids and GPI-anchored proteins to the Golgi complex.(Pelkmans and Helenius.Traf?c 2002;3:311–320.) Click for enlargement
Vol.3 Iss.4, April 2002	Cover Illustration: Different transport machineries of apical-and basolateral-like carriers in non-polarised cells? Double-transfected Vero cell according to Rustom et al. Traffic 2002;3:279–288. Shown is a maximum projection obtained from a dual-colour video sequence displaying the movement of hCgB-EYFP (yellow)and GPI-ECFP (b ue)containing carriers on their way from the TGN to the plasma membrane.By displaying the tracks (dotted lines)and dynamics (distance from one dot to the other)of these carriers,this kind of presentation clearly displays the apparent differences in their pattern of movement.Because in non-polarised cells hCgB-EYFP is selectively transported in basolateral- like carriers and GPI-ECFP is a speci ?c marker for apical-like carriers, this difference suggests the possible existence of diverse transport machineries of the respective types of carriers. Click for enlargement
Vol.3 Iss.3, March 2002	Cover Illustration: Differential interference contrast and uorescence image of GFP-expressing Salmonella typhimurium (green) within RAW 264.7 macrophages,12 hours after bacterial uptake. (Image courtesy of David Holden,Imperial College School of Medicine,London.)
Vol.3 Iss.2, Feb 2002	Cover Illustration: Lysosomal delivery in the developing Drosophila eye was analyzed by staining for the ligand Boss (red) which is expressed on the apical surface of R8 photoreceptor cells. All cells in this eye disc are mutant for the hook gene preventing detection of internalized Boss protein in R7 cells. The lack of the green marker identi.es a patch of cells also mutant for deep orange. In these cells, lysosomal delivery of Boss is inhibited and the ligand accumulates in endosomes in R7 cells (reference to review). Click for enlargement
Vol.3 Iss.1, Jan 2002	Cover Illustration: A Xenopus XLK2 cultured cell in late anaphase ?xed and stained for chromosomes (blue), microtubules (red)and Xenopus A rora-A kinase (green). Image is a projection through a 3D volume recorded using a DeltaVision Restoration Microscope (Applied Precision,Inc.) Image courtesy of JR Swedlow (see Traffc 2002;3(1):29 –36) Click for enlargement