Cover Legend: A tubular network of “Salmonella-induced filaments (Sifs)” in living HeLa cells expressing NPC1-eGFP, a marker for late endosomes/lysosomes, was imaged using confocal microscopy. This image was captured 9 hours after invasion by Salmonella typhimurium expressing mRFP. See article by Drecktrah et al. in this issue (Drecktrah et al. Traffic 2008;9(12):2117-2129).
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Cover Legend: Organelle transport in the squid giant axon. Left panel shows that organelles depleted of extraneous proteins continue to move at native rates on reconstituted microtubules. Center panel shows native organelles clinging to a native microtubule in axoplasm. Right panel shows the same organelles labeled by immunogold for Kinesin-3. This antibody also blocks the movement shown in the left panel, suggesting that Kinesin-3 is the motor for fast organelle transport in the squid giant axon. Image courtesy of J DeGiorgis (see DeGiorgis et al. Traffic 2008; 9(11):1867–1877).
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Cover Legend: Protein storage vacuoles of an Arabidopsis seed embryo, as highlighted by a tonoplast intrinsic protein-YFP fusion (green). A signal peptide-RFP reporter (red) is secreted and highlights the intercellular spaces. Image courtesy of Lorenzo Frigerio.
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Tubulovesicular secretory carriers in a neuronal dendrite.
Confocal imaging of a cultured hippocampal neuron expressing a GFP-tagged vesicular stomatitis virus G protein (VSVG-GFP). Fluorescence recovery after photobleaching (FRAP) after release of a temperature dependent blockade of trans Golgi network (TGN)-exit allows the visualization of anterograde (bottom to top) and retrograde (top to bottom) movements of dendritic tubulovesicular secretory carriers. Image courtesy of Ehlers and Hanus. See Traffic 2008;9:1437–1445.
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Cover Legend: Clathrin coated vesicles isolated from control and auxilin depleted HeLa cells. Auxilin depletion causes clathrin to form membraneless cages. See Hirst et al. Traffic 2008;9:1354–1371.
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Cover Legend: Muscles in Drosophila and Mammalian (C2C12) cell culture. (Top) Drosophila embryonic body wall muscles. This embryo is at stage 15 (~13 hours after egg laid), near the end of myoblast fusion, and carries the rp298-lacZ transgene. Muscles are labeled with phalloidin (red) and with antibody against Beta-galactosidase, which labels all muscle nuclei (green). (Bottom) Mammalian C2C12 myofibers in culture. Cells were allowed to differentiate and fuse into mytofibers for five days. Myofibers are labeled with phalloidin to visualize the actin cytoskeleton (red) and counterstained with DAPI (green) to visualize nuclei. Images are not to scale. Image courtesy of Mary Baylies (see Richardson et al. Traffic 2008;9:1050–1059).
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Cover Legend: 3D model of endothelial caveolae in situ Surface rendered model of a fast frozen endothelial cell in situ within a pancreatic islet of Langerhans, imaged by electron microscope tomography. The plasma membranes (green) contain abundant caveolae (highlighted and approximated by beige spheres) each with a diaphragm (blue). Microtubules (red) run close to the plasma membrane and caveolae (see Richter et al, Traffic 2008;9:893–909).
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Cover Legend: Life in vacuole. A parasitophorous vacuole containing 16 tachyzoites arranged in a rosette. The parasitophorous vacuole is stained for TgGRA3 (in red) containing Toxoplasma gondii tachyzoites stained for TgMIC6 (in green). (Image courtesy of Dominique Soldati-Favre. Reprinted with permission from Opitz et al Embo J., 2002.)
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Dynein-dependent microtubule sliding powers the translocation of the mitotic spindle across the bud neck in Saccharomyces cerevisiae. Timelapse images were captured on a spinning disk confocal microscope. Each frame represents a collapsed stack of 7 planes separated by 600 nm. Stacks were captured at 20 second intervals. Microtubules are labeled with GFP-tubulin (cyan), and the nucleus is labeled by a fusion of RFP to the nuclear pore complex component, Nup133 (magenta).  See Moore et al. Traffic 2008 9(4):510-527.
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Representation of a tomographic slice from a 3D-reconstruction of a HepG2 cell double-labelled for sortilin (indicated by green dots) and sorting nexin 1 (indicated by red dots). The picture outlines in blue an early endosomal vacuole that displays multiple so-called Endosome-to-TGN transport Carriers (ETCs). Sortilin and sorting nexin 1 colocalize to ETCs, which represent a novel type of tubular/vesicular carriers that directly bud from the early endosomal vacuole and specifically mediate retrograde transport of lysosomal protein sorting receptors. See Mari et al. Traffic 2008;9(3):380–393.
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The tangled mess of apicoplast and mitochondria development in Plasmodium falciparum: Plasmodium falciparum is the cause of the most severe form of human malaria. P. falciparum contains two endosymbiotic organelles that cannot be synthesised de novo. Within each replication cycle the mother cell must segregate these organelles into 18-36 daughter cells. This image shows the highly reticulated morphology of the apicoplast (in green) and the mitochondria (in red) during the late stages of intra-erythrocytic development in this deadly pathogen.  Image courtesy of C. Tonkin, see 166-175.
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A plant reticulon protein fused to YFP (red) localizes to the cortical endoplasmic reticulum (ER) in tobacco epidermal cells.
Its overexpression causes remodelling and constriction of the ER tubules, limiting protein diffusion within the ER lumen (highlighted by the soluble ER marker GFP-HDEL, green). See Tolley et al. Traffic 2008;9(1):94–102.
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