Volume 4 issue 12 December 2003
HIV-1 egress is gated through late endosomal membranes
Sascha Nydegger*, Michelangelo Foti†, Aaron Derdowski‡, Paul Spearman‡, Markus Thali*

*Department of Microbiology and Molecular Genetics, College of Medicine and CALS, 318 Stafford Hall, University of Vermont, Burlington, VT 05405, U.S.A.
†Department of Morphology, University of Geneva, CH-1211 Geneva, Switzerland
‡Department of Pediatrics and Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232, U.S.A.

Key words: retroviral assembly and release, late endosome, mulivesicular body (MVB), human immunodeficiency virus type 1 (HIV-1)

Running title: HIV-1 egress

Abbreviations: HIV, human immunodeficiency virus; VLP, virus-like particle; MVB, multivesicular body; MHC class II, major histocompatibility complex class II

Supplemental data for Fig. 1 Intracellular localization of HIV-1 in melanocytes. T-cell tropic HIV-1 (NL4-3) was expressed in Mel JuSo cells. Cells were analyzed after staining with patient serum and FITC-conjugated anti-human secondary antibody. Serial optical sections along the z axis are shown. Scale bar = 5 µm.

View Figure 1