Volume 5 issue 7 July 2004
Dense core vesicle dynamics in C. elegans neurons and the role of kinesin UNC-104
Tobias R. Zahn, Joseph K. Angleson, Margaret A. MacMorris, Erin Domke, John F. Hutton, Cindi Schwartz and John C. Hutton

Supplement1.mov1 : Laser scanning confocal image analysis of pharyngeal nerve ring of whole mount of wild type nematode stained with anti IDA-1 COOH terminal antibodies (initial orientation anterior down; dorsal left). Numerous isolated cell bodies are observed anterior and posterior to the intensely staining nerve ring that is comprised of a complex of neuronal cell bodies and their processes, many of which merge to form the ventral nerve cord.

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Supplement2.jpg : Epifluorescence microscopy of of a young adult hermaphrodite nematode showing expression of an IDA-1::GFP transgene driven by a 2443bp IDA-1 promoter (ventral lateral view; anterior to left. Of bilaterally symmetric pairs of cell bodies and neuronal processes only one partner is shown (except for PHC). 3D stacks of images were acquired (63X objective) and in-focus regions with GFP fluorescence were assembled to generate a composite image. The light intensity of some strongly fluorescent areas are scaled to a lower level to retain resolution.

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Supplement1.mov3 : PHC neuronal cell body and proximal dendrite (anterograde to right). Saltatory movement of an IDA-1::GFP-tagged vesicle is seen emerging from a perinuclear region into and through the posterior process. Time interval between frames: 180 ms. This Quicktime video corresponds to Fig. 3.

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Supplement1.mov4 : Movement of IDA-1::GFP-tagged vesicles in a PHC posterior process (anterograde upward). Time interval between frames: 201 ms. This Quicktime video corresponds to Fig. 6.

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(Quicktime videos can be accessed at www.uchsc.edu/misc/diabetes/ida-1movies.html)