Volume 6 issue 6 June 2005
HIV-1 Trafficking to the Dendritic Cell-T cell Infectious Synapse Uses a Pathway of Tetraspanin Sorting to the Immunological Synapse
Eduardo Garcia, Marjorie Pion, Annegret Pelchen-Matthews, Lucy Collinson, Jean-Francois Arrighi, Guillaume Blot, Florence Leuba, Jean-Michel Escola, Nicolas Demaurex, Mark Marsh and Vincent Piguet

SUPLEMENTARY MATERIAL:

Figure S1: Screening for HIV-1 intracellular compartment in LPS-mDC

LPS-mDCs were incubated at 37°C with HIV-1 to allow for internalization and analyzed by confocal microscopy. One representative cell in each condition is shown (Green: HIV-1-GFP; Red: cellular markers). Bar = 5 µm

Supplementary figure 1 (.jpg)

Figure S2: Screening for HIV-1 intracellular compartment in iDC and HIV-mDC

(A) iDCs were incubated at 37°C with HIV-1 for 2 hours (iDC) and for 24 hours (HIV-mDC) to allow for internalization and analyzed by confocal microscopy. One representative cell in each condition is shown (Green: Immunolabeling of HIV-1 p24^gag; Red: cellular markers, Blue: LAMP-1). Bar = 5 µm. (B) Fields of HIV-mDC were analyzed by immunofluorescence microscopy. (Green: Immunolabeling of HIV-1 p24^gag ; Red: cellular markers, Blue: DAPI (nucleus)).

Supplementary figure 2 (.jpg)

Figure S3: iDC immunofluorescence analysis

iDCs were analyzed by immunofluorescence microscopy. One representative cell in each condition is shown (Green: LAMP-1; Red: cellular markers, Blue: DAPI (Nucleus)). Bar = 5 µm.

Supplementary figure 3 (.jpg)

Supplemental Text:

Supplementary figure 4 (doc)