Volume 6 issue 7 July 2005
Analysis of the AP-2 adaptor complex and cargo during clathrin-mediated endocytosis.
J.Z Rappoport, A Benmerah, and S.M. Simon

SUPLEMENTARY MATERIAL:

 Supplementary figure 1: Clathrin and AP-2 at the sites of static and disappearing spots. The clathrin-dsRed and a -adaptin-EGFP intensities relative to local background were measured from 82 disappearing and static clathrin spots. A, the mean +/- SEM of intensity divided by local background, including subtraction of the number "1" to normalize for local background. B, the mean +/- SEM of the ratio of clathrin relative to local background divided by AP-2 relative to local background. (N.B. In both A and B the Y-axis begins at "1.0", local background.)

Supplementary figure 1 (.gif)

 Supplementary figure 2: Histograms of clathrin and AP-2 at the sites of static and disappearing spots. The distributions of the clathrin-dsRed and α-adaptin-EGFP intensities relative to local background are presented from 82 disappearing and static clathrin spots. A, histogram of AP-2 intensity divided by local background, including subtraction of the number "1" to normalize for local background. B, histogram of the ratio of clathrin relative to local background divided by AP-2 relative to local background.

Supplementary figure 2 (.gif)

 Video 1: Absence of AP-2 from disappearing clathrin spots. Images from video acquired at 240 ms/frame displayed at 10 frames/s demonstrate the disappearance of clathrin-dsRed without AP-2 (spots in center and bottom center of field).

Video 1 (.mov)

 Video 2: Colocalization of Tf in disappearing clathrin spots. Images from video acquired at 240 ms/frame displayed at 10 frames/s demonstrate the simultaneous disappearance of clathrin-dsRed and Alexa488-Tf (spot in center of field).

Video 2 (.mov)