Notch receptors are clustered and trans-endocytosed by Delta ligand cells. Confocal micrograph of a Delta expressing cell (left) interacting with a Notch expressing cell (right). Following interaction with Delta (blue), cell surface Notch (yellow) is clustered at cell-cell interfaces. Notch extracellular domain is detected within Delta cells (green) indicative of trans-endocytosis. Endocytosis of ligand while bound to Notch may produce a force sufficient to pull Notch apart and activate signaling.
 
 
 
 
 
 

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Volume 7 issue 12 December 2006
Citron Kinase, a RhoA Effector, Enhances HIV-1 Virion Production by Modulating Exocytosis
Rebecca J. Loomis, Derek A. Holmes, Andrew Elms, Patricia A. Solski, Channing J. Der and Lishan Su

Supplemental Figure 1: Citron kinase targeted short-hairpin RNA constructs. All citron kinase shRNA constructs were made in the pSUPER.Retro.Puro vector. The start site base pairs are from the beginning of the coding sequence. Italics = target sequence, Underline = hairpin.

Figure 1 (.jpg)

Supplemental Figure 2: Protein expression of citron kinase mutants. (A, B) Expression of citron constructs was verified by analyzing cell-associated proteins with α-Myc, α-p24 and α-actin antibodies. All citron constructs are Myc-tagged at the N-terminus.

Figure 2 (.jpg)

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