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Volume 7 issue 4 April 2006
Differential Properties of GTP- and Ca2+-stimulated Exocytosis from Large Dense Core Vesicles
Li Bai, Dan Zhu, Keming Zhou, Wei Zhou, Dongdong Li, Yan Wang, Rongying Zhang and Tao Xu
Supplementary Figure Legend:
Supplementary Figure 1. Cumulative numbers of fusion events stimulated by mastoparan were plotted against time from cells with (TG+Mas, open circle) or without (Mas, filled circle) pretreatment of thapsigargin (TG, 2 µM for 15 minutes). Also compared are mastoparan-induced fusion events from cells pretreated with Latrunculin B (5 µM) for 5 minutes (LB+Mas).
Figure 1 (.tif)
Supplementary Figure 2. Tentative model for the Ca2+ and GTP-dependent exocytosis. Ca2+ and activated G-proteins control exocytosis via two different mechanisms. G-protein activation triggers translocation and docking of vesicles and subsequent transient fusion. Through a brief opening of the fusion pore, only a small amount of content is released. Whereas the primed vesicles are spared by GTP, they are triggered to fuse by Ca2+. Fusion of LDCV induced by Ca2+ has a longer and larger opening of the fusion pore, which results in larger content release and also partial diffusion of certain vesicular membrane proteins.
Figure 2 (.jpg)
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