Volume 7 issue 6 June 2006
P-selectin and CD63 use different mechanisms for delivery to Weibel-Palade bodies
Kimberly J Harrison-Lavoie, Grégoire Michaux, Lindsay Hewlett, Jasber Kaur, Matthew J Hannah, Winnie W Y Lui-Roberts, Keith E Norman and Daniel F Cutler

Figure S1 shows individual channels for Figure 1 to clarify the targeting of ssHRP-P-selectin and mutants to Weibel-Palade bodies.

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Figure S2. Raw data for Figure 1 graph.

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Figure S3. EM analysis of HRP-PselWT.
HUVEC were microinjected (A, C) or nucleofected (B) to express HRP-PselWT, and then left for 4h (A), or 25h (B) or 24h (C) before processing for EM. Bars are 100nm.
Note the accumulation of material within the highly fenestrated structures adjacent to the Golgi stacks, most likely the TGN.

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Figure S4. EM analysis of HRP-PselKCPL.
HUVEC were nucleofected to expressHRP-PselKCPL, and then left for 5h (A), or 15h (B) before processing for EM. Bars are 100nm.
Note the accumulation of material within structures adjacent to the Golgi stacks, most likely the TGN.

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Figure S5. EM analysis of HRP-PselYGVF.
HUVEC were nucleofected to express HRP-PselYGVF, and then left for 5h (A), or 15h (B, C) before processing for EM. Note the complete lack of staining within the TGN. Scale bars are 100nm.

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Videos. Videos relating to Figure 4 showing leukocyte rolling in Pearl (Figure 4video1.mov), Pearl plus anti P-selectin antibody (Figure 4video2.mov) and Wild-type (Figure 4video3.mov) mice.