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Volume 7 issue 7 July 2006
Identification of an intracellular trafficking and assembly pathway for HIV-1 Gag
Mira Perlman and Marilyn D. Resh
Supplemental Figure 1. VLP production and stability of GagTC compared to Gag. COS-1 ( A,B ) or HeLa ( C ) cells were transfected with pCMV5-Gag or pCMV5-GagTC. (A) COS-1 cells were labeled with Tran35S-label for varying lengths of time and VLP production was quantitated as described in Material and Methods. ( B ) COS-1 cells were pulse-labeled for 7 min with Tran35S-label and incubated in chase medium for the indicated lengths of time. The amount of Gag in the cells and in extracellular VLPs was determined by immunoprecipitation with anti-p24 antibody and autoradiography. ( C ) HeLa cells transfected with pCMV5-Gag or pCMV5-GagTC were analyzed for Gag stability as in ( A ). Closed circles = Gag-TC; closed squares = Gag.
Figure 1 (.tif)
Supplemental Figure 2. Perinuclear GagTC and anti-MA antibody staining overlap. The goat anti-MA antibody staining (blue) for the HeLa cells depicted at the 1 hr (A) and 2 hr (B) time points in Figure 2 is shown along with the FlAsH and ReAsH signals. Note that all of the red staining overlaps with the green and blue signals to generate white (arrows). Bars represent 5 µm.
Figure 2 (.tif)
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