Volume 7 issue 8 August 2006
Vesicular trafficking of semaphorin 3A is activity-dependent and differs between axons and dendrites
Joris de Wit, Ruud F. Toonen, Joost Verhaagen and Matthijs Verhage

Supplementary Movies

Movie 1
GFP-Sema3A trafficking in an axon of a cortical neuron (17 DIV). Movie shows a 1 minute time-lapse sequence, with images taken every second. Movie frames have been inverted for clarity. Cell body located to the left outside the picture frame. Scale bar 5 µm.

Movie 1 (.mov)

Movie 2
GFP-Sema3A trafficking in a dendrite of a cortical neuron (14 DIV). Movie shows a 1 minute time-lapse sequence, with images taken every second. Movie frames have been inverted. Cell body located to the left outside the picture frame. Scale bar 5 µm.

Movie 2 (.mov)

Movie 3
GFP-Sema3A trafficking in an axon (DIV 15) before, during and after application of a 30s depolarizing (60 mM KCl) stimulus. Movie shows a 3 minute time-lapse sequence, with images taken every second. Cells were imaged for 30s, followed by a 30s depolarizing stimulus. Cells were imaged for an additional 2 minutes after the stimulus had ended. Duration of stimulus is indicated in the movie. Movie frames have been inverted. Cell body located to the left outside the picture frame. Scale bar 10 µm.

Movie 3 (.mov)