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Volume 8 issue 10 October 2007
rAAV6-Microdystrophin Rescues Aberrant Golgi Complex Organization in mdx Skeletal Muscles
Justin M. Percival, Paul Gregorevic, Guy L. Odom, Glen B. Banks, Jeffrey S. Chamberlain and Stanley C. Froehner
Supplemental Figure 1: Fiber type-specific organization of the synaptic Golgi complex. Golgi organization was evaluated at the neuromuscular junctions (NMJs) of gastrocnemius (A-D) and soleus myofibers (E-H). NMJs were identified using TRITC-conjugated α-bungarotoxin which specifically binds the acetylcholine receptor (AChR) at postsynaptic membranes (B and F). In gastrocnemius muscles, the GC is concentrated at the synapse (A) and localized around nuclei in a perinuclear distribution (A, C and D). GCEs were concentrated around the edges of the synapse (A, arrowheads). Non-muscle cells with ribbon-like GC associated with wild type NMJs (A, C and D, arrow). AChRs did not localize to the Golgi complex (A, B and D). At the soleus NMJ, the spatial arrangement of the GC was completely different to the synaptic GC of gastrocnemius myofibers (E). There were more nuclei at synapses in soleus myofibers (G cf. C). The GC were sometimes found in a perinuclear localization at NMJs of soleus myofibers (E, G and H). Fiber-specific mechanisms regulate the organization of the GC at the neuromuscular synapse. Scale bar: 8 µm.
Figure 1 (.jpg)
Supplemental Table 1: Synaptic Golgi complex organization in wild type soleus and gastrocnemius myofibers. Synapse area (µm2), GC density (GC puncta / 1000 µm2), GC area (µm2), and GC circularity, the number of nuclei per NMJ and the number of nuclei with perinuclear GC were compared between junctions from wild type soleus and gastrocnemius myofibers. Although the localization of the GC was different (Supplementary Data: Figure 1), there was no significant difference between any of the other characteristics of the GC between wild type junctions of soleus and gastrocnemius myofibers. However, there were more nuclei at the synapses of soleus muscle cells. Arrows pointing downwards represent a decrease and arrows pointing upwards represent an increase. Mean values ± standard error of the mean are reported. *: p < 0.01. n.s: not statistically significant.
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Soleus |
Gastrocnemius |
% Difference |
NMJ Area (µm2) |
368.16 ± 23.83 |
307.91 ± 14.11 |
↓16 %n.s |
GC Density |
10.83 ± 0.69 |
15.51 ± 0.82 |
↑43 %n.s |
Nuclei per NMJ |
12.2 ± 0.56 |
7.71 ± 0.34 |
↓37 %* |
Nuclei with Perinuclear GC |
0.72 ± 0.17 |
0.83 ± 0.16 |
↑15 %n.s |
GC Area (µm2) |
0.34 ± 0.02 |
0.44 ± 0.01 |
↑29 %n.s |
GC Circularity (0-1) |
0.23 ± 0.02 |
0.4 ± 0.02 |
↑74 %n.s |
*: p < 0.01, n.s: not statistically significant. |
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