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Volume 8 issue 11 November 2007
Homodimerization antagonizes nuclear export of Survivin
D Engelsma, JA Rodriguez, A Fish, G Giaccone and M Fornerod
Supplemental Figure 1: Influence of NES mutation, cell line and N or C-terminal YFP-tagging on the subcellular localization of Survivin. (A) Subcellular localization of YFP-SurvivinL96A/L98A in MCF7 and HeLa cells. (B) Subcellular localization in MCF7 cells of YFP-SurvivinL96A and SurvivinL96A-YFP. Bar, 10 µm.
Figure 1 (.jpg)
Supplemental Figure 2: Influence of expression level on nucleocytoplasmic ratios of Survivin(1-109) and Survivin(1-142), carrying or not carrying the F101A/L102A mutation. Expression levels are approximated by cytoplasmic pixel intensities in arbritrary units (AU). Coefficients (coef) are from least sum of squares linear fits. For Survivin(1-109) the data is from one of two experiments also shown in Fig 5B.
Figure 2 (.jpg)
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