Volume 8 issue 11 November 2007
A membrane protease is targeted to the relict plastid of Toxoplasma via an internal signal sequence
Anuradha Karnataki, Amy E. DeRocher, Isabelle Coppens, Jean E. Feagin, and Marilyn Parsons

Supplemental Figure 1: Alignment of TgFtsH1 with the PfFtsH1(PFL1925w), a chloroplast FtsH-Arabidopsis FtsH1 (Q39102), a mitochondria FtsH-Saccharomyces cerevisiae Yta12p (NP_013807), and a bacterial FtsH-Baizongia pistaciae (Q89AF2). Alignment of TgFtsH1 with the PfFtsH1(PFL1925w), a chloroplast FtsH-Arabidopsis FtsH1 (Q39102), a mitochondria FtsH-Saccharomyces cerevisiae Yta12p (NP_013807), and a bacterial FtsH-Baizongia pistaciae (Q89AF2) is shown. Black shading marks residues identical in all sequences, while dark and light gray shading mark residues identical or similar in at least 3 sequences, respectively. Conserved residues in Walker A (GTK- bold and blue), Walker B (DE- bold and pink), SRH (RXXR- bold and green), Zn binding (HEAGH- bold and yellow) are highlighted.Transmembrane domains predicted by either TMHMM or TMpred and are shown in red, bold and italics. Arrows indicate the C-termini of the FtsH deletion mutants (354, 694 and 925).The strain specific amino acid changes from the ME49 are at location 1008, 1024, 1194 and 1222.

Figure 1 (.pdf)

Supplemental Table 1: Oligonucleotides.

Table 1 (.pdf)