Volume 8 issue 2 February 2007
Sterol, protein and lipid trafficking in Chinese Hamster Ovary cells with
Niemann-Pick type C1 defect
Nina H. Pipalia, Mingming Hao, Sushmita Mukherjee, Frederick R. Maxfield

Figure S1. Trafficking of DHE and Tf in normal (25RAhTfR) and NPC1 (CT60hTfR) cells. Normal (25RAhTfR) and NPC1 (CT60hTfR) cells were pulse labeled with DHE and chased for various periods of time as indicated. The ERC in these cells were identified by labeling the cells with Alexa488-Tf during the final 30 min of chase and chasing out the labeled Tf from the PM and SE with excess unlabeled Tf and deferoxamine. Alexa488-Tf (A, D, G, J, M, P, S, V), DHE (B, E, H, K, N, Q, T, W) and the color overlay images of both fluorophores (C, F, I, L, O, R, U, X) are displayed, as indicated at the top of each column. All images were acquired live using a 63X objective.
Bar: 10 µM.

Figure 1(.jpg)