Notch receptors are clustered and trans-endocytosed by Delta ligand cells. Confocal micrograph of a Delta expressing cell (left) interacting with a Notch expressing cell (right). Following interaction with Delta (blue), cell surface Notch (yellow) is clustered at cell-cell interfaces. Notch extracellular domain is detected within Delta cells (green) indicative of trans-endocytosis. Endocytosis of ligand while bound to Notch may produce a force sufficient to pull Notch apart and activate signaling.
 
 
 
 
 
 

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Volume 8 issue 2 February 2007
The Drosophila Pigmentation Gene pink (p) Encodes a Homolog of Human Hermansky-Pudlak Syndrome 5 (HPS5)
Juan M. Falcón-Pérez, Rafael Romero-Calderón, Elizabeth S. Brooks, David E. Krantz
and Esteban C. Dell'Angelica

Supplemental figure S1: Phylogenetic analysis of the Drosophila CG9770 gene product and related protein sequences. (A) Alignment of the predicted amino acid sequences of human HPS5 (isoform a; GenBank NP_852608) and the fruit fly ( Drosophila melanogaster ) CG9770 gene product (CG9770-PA; GenBank NP_649810). Identical amino acid residues are highlighted. Numbers on the right indicate residue positions. The mutant sequence created by frameshift in the fly lines p 1, p p and psnb is underlined; the asterisk denotes an early termination codon. (B) Unrooted tree depicting the phylogenetic relationships between the two protein sequences shown in (A) as well as mouse ( Mus musculus ) HPS5 isoform 1 (GenBank NP_001005247), chicken ( Gallus gallus ) HPS5-like protein (GenBank XP_421011), zebrafish ( Danio rerio ) LOC560531 protein (GenBank AAI21197), red flour beetle ( Tribolium castaneum ) CG9770-PA-like protein (GenBank XP_975447), human hypothetical protein LOC9895 (also known as protein KIAA0297/KIAA0329; GenBank NP_055659), mouse hypothetical protein LOC104859 isoform 1 (GenBank XP_127142), chicken LOC9895-like protein (also known as similar to hypothetical protein KIAA0297/KIAA0329; GenBank XP_421376), zebrafish hypothetical protein LOC569212 (GenBank NP_001038644), red flour beetle CG11141-PB-like protein isoform B (GenBank XP_969871) and fruit fly CG11141-PA protein (GenBank NP_724559; the predicted amino acid sequences of CG11141-PA and -PB are identical). The numbers next to branching points indicate bootstrap values resulting from 100 re-sampling cycles.

Figure 1(.gif)

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