Notch receptors are clustered and trans-endocytosed by Delta ligand cells. Confocal micrograph of a Delta expressing cell (left) interacting with a Notch expressing cell (right). Following interaction with Delta (blue), cell surface Notch (yellow) is clustered at cell-cell interfaces. Notch extracellular domain is detected within Delta cells (green) indicative of trans-endocytosis. Endocytosis of ligand while bound to Notch may produce a force sufficient to pull Notch apart and activate signaling.
 
 
 
 
 
 

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Volume 8 issue 3 March 2007
Oligomerization is a specific requirement for apical sorting of GPI-anchored proteins but not for non-raft associated apical proteins
Simona Paladino, Daniela Sarnataro, Simona Tivodar, and Chiara Zurzolo

Figure S1. DPPIV is not present in cross-linkable complexes on the apical membrane, while Na,K-ATPase is in an oligomeric complex at the basolateral surface. BS3 (0.5 mM) was added on the apical or basolateral surface of FRT cells grown on filters. After lysis, proteins were TCA-precipitated, run on SDS-PAGE (10% polyacrylamide) and revealed by western blots using specific antibody against DPPIV [ generous gift from Dr. A. Quaroni ( Cornell University , Ithaca , New York ); Quaroni and Isselbacher,1985] and against the subunit α1 of Na,K-ATPase ( from Santa Cruz Biotechnology). The molecular weight of the monomeric forms (*) of each protein is indicated, together with the position of standard markers.

Quaroni A, and Isselbacher KJ . Study of intestinal cell differentiation with monoclonal antibodies to intestinal cell surface components. Dev Biol. 1985 Oct;111(2):267-79.

Figure 1(.jpg)

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