Volume 8 issue 3 March 2007
The interaction of two tethering factors, p115 and COG complex, is required for Golgi integrity
M Sohda, Y Misumi, S Yoshimura, N Nakamura, T Fusano, S Ogata, S Sakisaka and Y Ikehara

Figure S1: Maintenance of the Golgi structure requires the HR2 domain of p115. A) ΔHR2 of p115 caused irregular Golgi reassembly. Plasmid with or without (mock) wild-type (WT), or ΔHR2 p115 cDNA was injected into nuclei of p115 KD cells using Cascade Blue-dextran as an injection marker after 91 h of siRNA treatment. After 5 h of incubation, the cells were fixed and double-labeled with antibodies for p115 and giantin. Cells were imaged by using the confocal microscope with x 63 objective. Stained images were presented as projections of stacked image with 14 sections of 0.42 µm thickness sections. Bars represent 20 µm. B) Morphology of the reassembled Golgi in WT or ΔHR2 p115 injected p115 KD cells, shown as enlarged view of the Golgi apparatus in A stained with giantin. Stained images were presented as a section of 0.42 µm thickness using the confocal microscope with x 63 objective.

Figure 1(.jpg)