Volume 8 issue 4 April 2007
Low temperature-induced Golgi tubules are transient membranes enriched in molecules regulating intra-Golgi transport
E Martínez -Alonso, J Ballesta, J A Martínez-Menárguez

Figure 1:Live imaging of CFP-GalTr. Supplement to Fig. 5A. HeLa cells expressing CFP-GalTr were cultured at 15ºC and imaged in the xyz directions every 20 seconds for 1000 seconds. Movie is x100 accelerated relative to real time. Example of retracting-type Golgi tubule.

Video 1 (.mov)

Figure 2. Live imaging of CFP-GalTr. Supplement to Fig. 5B. HeLa cells expressing CFP-GalTr were cultured at 15ºC and imaged in the xyz directions every 20 seconds for 1600 seconds. Movie is x100 accelerated relative to real time. Example of detaching-type Golgi tubule.

Video 2 (.mov)

Figure 3. Live imaging of CFP-GalTr. Supplement to Fig. 5C. HeLa cells expressing CFP-GalTr were cultured at 37ºC and imaged in the xyz directions every 20 seconds for 840 seconds. Movie is x65 accelerated relative to real time. Example of detaching-type Golgi tubule.

Video 3 (.mov)

Figure 4. Live imaging of CFP-GalTr. Supplement to Fig. 5D. HeLa cells expressing CFP-GalTr were cultured at 37ºC and imaged in the xyz directions every 10 seconds for 495 seconds. . Movie is x80 accelerated relative to real time. Example of retracting-type Golgi tubule.

Video 4 (.mov)