Volume 8 issue 5 May 2007
Molecular identification of 26 syntaxin genes and their assignment to the different trafficking pathways in Paramecium
Roland Kissmehl, Christina Schilde, Thomas Wassmer, Carsten Danzer, Kathrin Nuehse, Kaya Lutter, and Helmut Plattner

Figure 1: Treatment of cells with brefeldin A had no effect on the subcellular localisation of Pt Syx2 in the contractile vacuole system. Cells were treated with brefeldin A, fixed and permeabilised as described in Materials and Methods. Immunostaining was performed with an affinity-purified anti-Syntaxin2 rabbit antibody and secondary AlexaFluor ® 488 coupled goat-anti-rabbit (Molecular Probes). No difference in the staining pattern
of Pt Syx2 was found between control cells treated with the same concentration of solvent and brefeldin A-treated cells. Bar = 10 µm.

Figure 1 (jpg)

Figure 2:

Table A: Oligonucleotides used to study syntaxin gene expression (cDNA)

Table B: Oligonucleotides used to amplify syntaxin for GFP tagging at the C-terminus

(C) or N-terminus (N)

Table C: Oligonucleotides for heterologous expression of His-tagged Pt Syx1-1 peptide

I₈₂ - I₂₁₀ in E. coli

Figure 2 (.doc)