Notch receptors are clustered and trans-endocytosed by Delta ligand cells. Confocal micrograph of a Delta expressing cell (left) interacting with a Notch expressing cell (right). Following interaction with Delta (blue), cell surface Notch (yellow) is clustered at cell-cell interfaces. Notch extracellular domain is detected within Delta cells (green) indicative of trans-endocytosis. Endocytosis of ligand while bound to Notch may produce a force sufficient to pull Notch apart and activate signaling.
 
 
 
 
 
 

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Volume 8 issue 5 May 2007
Regulation of phagocytosis in Dictyostelium by the inositol 5-phosphatase OCRL
homologue Dd5P4

HM Loovers, A Kortholt, H de Groote, L Whitty, RL Nussbaum and PJM van Haastert

Figure 1: Confocal fluorescent images of AX3 wild type cells and dd5p4-null cells expressing Dd5P4-GFP or the catalytic inactive mutant Dd5P4D319A-GFP. The field of observation is 100 x 100 µm.

Figure 1 (jpg)

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