Volume 8 issue 5 May 2007
Evidence for a direct role of the Doa4 deubiquitinating enzyme in protein sorting into
the MVB pathway
E Nikko  and B André

Figure 1: Gap1 was detected by Western blotting in total protein extracts prepared before (t = 0 min) and at the indicated times after addition of NH4⁺. Strains and growth conditions were as in Fig. 5A.

Figure 1 (jpg)

Figure 2: Upper part. Gap1 activity was measured before (t = 0 min) and at several times after 20mM NH4⁺-addition, as in Fig. 1B, in wild-type (23344c, ■) and snƒ7Δ cells(ME029, □), and in snƒ7Δ cells transformed with the ubiquitin-gene-bearing YEp96 plasmid (Ο) and overproducing ubiquitin by addition of CuSO₄ (100 µM) to the medium. Bottom part. As in the upper figure, except that vps4Δcells (ME27) were used instead of snƒ7Δ cells.

Figure 2 (jpg)

Figure 3: Gap1 was detected by Western blotting in total protein extracts prepared before (t = 0 min) and at the indicated times after addition of NH4⁺. Strains and growth conditions were as in Fig. 5A.

Figure 3 (jpg)

Figure 4: Gap1 was detected by Western blotting in total protein extracts prepared before (t = 0 min) and at the indicated times after addition of NH4⁺. Strains and growth conditions were as in Fig. 5A.

Figure 4 (jpg)