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Volume 8 issue 6 June 2007
A MICROTUBULE-FACILITATED NUCLEAR IMPORT PATHWAY FOR CANCER REGULATORY PROTEINS
Daniela Martino Roth1,2, Gregory W. Moseley1, Dominic Glover1, Colin W. Pouton2 and David A. Jans1,*
Figure 4. Nocodazole treatment reduces the rate and extent of Rb nuclear import as determined using FRAP.
Movie 1:This movie shows the return of nuclear fluorescence in an untreated COS-7 cell transiently expressing GFP-Rb after photobleaching. A small region of the nucleus in this cell is photobleached and subsequent images were collected at 20 s intervals for 580s.
Video 1 (zipped mov)
Movie 2: This movie shows the return of nuclear fluorescence in an NCZ-treated COS-7 cell transiently expressing GFP-Rb after photobleaching. A small region of the nucleus in this cell is photobleached and subsequent images were collected at 20 s intervals for 580s.
Video 2 (zipped mov)
Figure 5. Nocodazole treatment does not affect the rate or extent of SV40 T-antigen nuclear import as determined using FRAP
Movie 3: This movie shows the return of nuclear fluorescence in an untreated COS-7 cell transiently expressing GFP-T-ag after photobleaching. A small region of the nucleus in this cell is photobleached and subsequent images were collected at 20 s intervals for 580s.
Video 3 (zipped mov)
Movie 4: This movie shows the return of nuclear fluorescence in an NCZ-treated COS-7 cell transiently expressing GFP-T-ag after photobleaching. A small region of the nucleus in this cell is photobleached and subsequent images were collected at 20 s intervals for 580s.
Video 4 (zipped mov)
Figure 6. Nocodazole treatment does not affect the rate and extent of Rb nuclear export determined using FRAP.
Movie 5: This movie shows the return of cytoplasmic fluorescence in an untreated COS-7 cell transiently expressing GFP-Rb after photobleaching. A region comprising 60 % of the cytoplasm in this cell is photobleached and subsequent images were collected at 20 s intervals for 460s.
Video 5 (zipped mov)
Movie 6: This movie shows the return of cytoplasmic fluorescence in an NCZ-treated COS-7 cell transiently expressing GFP-Rb after photobleaching. A region comprising 60 % of the cytoplasm in this cell is photobleached and subsequent images were collected at 20 s intervals for 460s.
Video 6 (zipped mov)
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