Volume 8 issue 7 July 2007
Rab27b regulates mast cell granule dynamics and secretion
K Mizuno, T Tolmachova, DS Ushakov, M Romao, M Åbrink, MA Ferenczi, G Raposo and MC Seabra

Supplemental Figure 1: Toluidine blue staining of skin sections.
Dermal mast cells were detected with toluidine blue staining in double heterozygote, Rab27a KO, Rab27b KO and double KO mice back dermis. The sections were also stained with eosin. The highly metachromatic cells are mast cells. Arrowhead, mast cell. Bar, 200 µm. 

Figure 1 (.jpg)

Supplemental Video 1: Secretory granule dynamics in double heterozygote BMMCs.
Images were captured every second.  Playback speed is 7 frames per second. See Figure 6 for more details.

Video 1 (.mov)

Supplemental Video 2: Secretory granule dynamics in double Rab27 KO BMMCs.
Imageswere captured every second.  Playback speed is 7 frames per second. See Figure 6 for more details.

Video 2 (.mov)

Supplemental Video 3: Secretory granule dynamics in double Rab27 KO BMMCs treated
with nocodazole.
Images were captured every second.  Playback speed is 7 frames per second. See Figure 6 for more details.

Video 3 (.mov)