Notch receptors are clustered and trans-endocytosed by Delta ligand cells. Confocal micrograph of a Delta expressing cell (left) interacting with a Notch expressing cell (right). Following interaction with Delta (blue), cell surface Notch (yellow) is clustered at cell-cell interfaces. Notch extracellular domain is detected within Delta cells (green) indicative of trans-endocytosis. Endocytosis of ligand while bound to Notch may produce a force sufficient to pull Notch apart and activate signaling.
 
 
 
 
 
 

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Volume 8 issue 8 August 2007
Myotubularin Lipid Phosphatase binds the hVPS15/hVPS34 Lipid Kinase Complex on Endosomes
Canhong Cao4, Jocelyn Laporte2, Jonathan M. Backer3, Angela Wandinger-Ness4,5 and Mary-Pat Stein1

Supplemental Figure 1.  MTM1 and Rab5Q79L are in discrete domains on extensively dilated endosomes.  BHK cells were transfected with MTM1C375S-FLAG and Rab5Q79L.  Cells were permeabilized with saponin, fixed and stained as described in Materials and Methods. MTM1C375S-FLAG was detected with a mAb directed against the FLAG epitope and a FITC-conjugated donkey anti-mouse IgG.  EEA1 was stained with human anti-EEA1 and a Texas Red-conjugated donkey anti-human IgG.  Rab5 was localized using a rabbit pAb directed against Rab5 and Cy5-conjugated donkey anti-rabbit IgG.  Insets are enlarged 2.2 times.  Bar 5 µm.

Figure 1 (.jpg)

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