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Volume 8 issue 8 August 2007
A di-acidic sequence motif enhances the surface expression of the potassium channel TASK-3
Marylou Zuzarte, Susanne Rinné, Günter Schlichthörl, Andrea Schubert, Jürgen Daut and Regina Preisig-Müller
Legend of Supplemental Figure S1
Supplemental Figure 1: Wild-type TASK-3 (with the EDE motif) and the mutant TASK‑3 in which the EDE motif was replaced by ADA were expressed in Xenopus oocytes and their pH sensitivity was compared. The data were fitted with the equation
I = 1 / [ 1 + (10(pK – pH))h ]
where I is the relative current amplitude, pK is the pH value corresponding to half-maximal inhibition of the current, and h is the Hill coefficient. For wt TASK-3, pK was 6.09 ± 0.03 and h was 1.036 ± 0.075 (n = 5); for the ADA mutant pK was 6.17 ± 0.06 and h was 1.100 ± 0.156 (n = 5); these values were not significantly different. Thus, the mutation had no significant effect on the pH sensitivity of the channels.
Figure 1 (.jpg)
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