Volume 8 issue 9 September 2007
Intracellular trafficking of potato leaf roll virus movement protein in transgenic Arabidopsis
F Vogel, D Hofius and U Sonnewald

Supplemental Figure 1: Long-term effect of Brefeldin A on Golgi bodies and ER structure. (A) MAN1:YFP labeled Golgi bodies; (B) Aggregated Golgi stacks after 10 µg/ml Brefeldin A (BFA) treatment for 72 hours; (C, D) Treatment of HDEL:CFP expressing Arabidopsis plants with or without BFA for 72 hours; (C) ER in source leaves shows a reticulate network after control treatment with 0,1 % DMSO; (D) This reticulate network is still visible after treatment with BFA; Bar = 20 µm.

Figure 1 (.jpg)

Supplemental Figure 2: Effects of Latrunculin B and Clasto lactacystin ß-lactone on the localization of soluble GFP. CLSM images show superimpositions of GFP-fluorescence (green) and propidium iodide fluorescence of stained cell walls (red); (A – D) Transgenic Arabidopsis plants expressing soluble GFP under control of the companion cell specific promoter AtSUC2 were treated with either 5 µM Latrunculin B (LatB) or 20 µM Clasto lactacystin ß-lactone (CLL). (A, B) Soluble GFP is dispersed in the cytoplasm in epidermal cells of source leaves without (A) or with LatB treatment for 5 d (B); (C, D) GFP is localizing to the cytoplasm in sink leaves (C) and no aggregations of GFP were visible in response to incubation with CLL for 8 hours; Bar = 20 µm.

Figure 2 (.jpg)