Volume 1 Issue 11 November 2000
Actin Assembly at the Membrane controlled by ARF6
Schafer and Cooper

Movie 1a.
Actin assembly at foci on the ventral surface of control, mock-injected PtK1 cell expressing GFP-CP. The video was collected using a confocal microscope focused at the ventral cell surface. The rate of playback is ~25 times real time. The scale bar is 5 µm.

Movie1a.mov

Movie 1b.
Actin assembly at foci on the ventral surface visualized using GFP-CP in a cell expressing ARF6(Q67L). The video was collected using a confocal microscope focused at the ventral cell surface. The rate of playback is ~25

Movie1b.mov

Movie 1c.
Actin assembly at foci on the ventral surface visualized using GFP-CP in a cell expressing ARF6(T27N). The video was collected using a confocal microscope focused at the ventral cell surface. The rate of playback is ~25 times real time.

Movie1c.mov

Movie 3a.
Particles with trailing tails of GFP-CP move in cells expressing ARF6(Q67L). Many of the particles form and move away from a juxtanuclear site. The scale bar is 5 µm and the video is shown at 10 times real time.

Movie3a.mov

Movie 3c.
Some moving particles appear as vesicles, based on the cup-shaped morphology at the leading tip of the GFP-CP tail.

Movie3c.mov