Supp. Mat. Volume 2 Issue 3 March 2001 - Traffic the International Journal of Intracellular Transport

Notch receptors are clustered and trans-endocytosed by Delta ligand cells. Confocal micrograph of a Delta expressing cell (left) interacting with a Notch expressing cell (right). Following interaction with Delta (blue), cell surface Notch (yellow) is clustered at cell-cell interfaces. Notch extracellular domain is detected within Delta cells (green) indicative of trans-endocytosis. Endocytosis of ligand while bound to Notch may produce a force sufficient to pull Notch apart and activate signaling.

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Volume 2 Issue 3 March 2001
Secretory and endocytic pathways converge in a dynamic endosomal system in a primitive protozoan
E Ghedin, A Debrabant, JC Engel and DM Dwyer

Time-lapse images demonstrating the dynamics of the endosomal tubular compartment in a transfected Leishmania major promastigote. The parasite, expressing a GFP chimeric protein that accumulates in the tubule, was imaged by confocal microscopy every 10 s over a period of 20 min.

MSTR90140_Movie.mov