Volume 2 Issue 5 May 2001, pages 277-298
Vesicular and nonvesicular transport of phosphatidylcholine in polarized HepG2 cells
D Wüstner, S Mukherjee, FR Maxfield, P Müller and A Herrmann

Video sequence to Fig. 7.
Vesicle movement in HepG2 cells labeled with b-BODIPY-PC. HepG2 cells were labeled for 2 min at 37°C with b-BODIPY-PC and after washing further incubated for 30 min at 37°C. Subsequently, images were acquired with a cooled CCD-camera every minute for a total time of 20 min on a temperature-controlled microscope stage (34-36°C). In the two cells in the center of the field, which form a ring-like BC, several types of vesicular movements were observed. Within 3 min of beginning the imaging sequence, a vesicle appeared sub-apically in the right cell and fused with the BC as indicated by its disappearance in the absence of any focal plane drift. Moreover, within 10 min of imaging, a vesicle was seen to move from the basolateral membrane domain to the SAC/ARC. Additionally bidirectional vesicle traffic between the SAC/ARC and the plasma membrane in these polarized cells and between the PNC and the plasma membrane in adjacent non-polarized HepG2 cells was observed. The time sequence is played with 6 frames per second.

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