Notch receptors are clustered and trans-endocytosed by Delta ligand cells. Confocal micrograph of a Delta expressing cell (left) interacting with a Notch expressing cell (right). Following interaction with Delta (blue), cell surface Notch (yellow) is clustered at cell-cell interfaces. Notch extracellular domain is detected within Delta cells (green) indicative of trans-endocytosis. Endocytosis of ligand while bound to Notch may produce a force sufficient to pull Notch apart and activate signaling.
 
 
 
 
 
 

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Volume 2 Issue 5 May 2001, pages 336-346
The GRIP Golgi localisation domain of p230 is a specific targeting sequence for a population of trans-Golgi network derived vesicles
DL Brown, K Heimann, L Kjer-Nielsen, C van Vliet, JL Stow and PA Gleeson

Movie 1. H10 Control
Real-time imaging of GFP-p230-GRIP in H10 cells. Live H10 cells were viewed under epifluorescence and images captured at 5 second intervals for 10 minutes and animated using NIH image. GFP-p230-GRIP localizes to the Golgi complex and to highly dynamic tubules emerging there-from. Some tubules or vesicular tips reach almost to the cell periphery.

View movie
H10-ctrl.mov

Movie 2. H10- Nocodazole
Real-time imaging of H10 cells treated with Nocodazole. Immediately following addition of the drug, cells were viewed under epifluorescence and images captured at 17 second intervals for 23 minutes. The labelled Golgi complex scatters and long tubules are no longer seen emerging. The labelled structures gradually fragment further.

View movie
H10-Noc.mov

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