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Volume 3 issue 11 November 2002, p. 791
Endosome Fusion and Microtubule-based Dynamics in the Early Endocytic Pathway of Dictyostelium
Margaret Clarke, Jana Köhler, John Heuser, and Günther Gerisch
Movie 1. Early processing of a macropinosome labeled with TRITC-dextran. For details, see legend to Figure 1. Frame-to-frame intervals were 5 seconds.
Video 1
Movies 2 and 3. The mixing compartment in cells labeled first with green fluorescent dextran (for 17 minutes starting at T = 0) and then with red fluorescent dextran (starting at T = 20 minutes). Movie 2 begins at T = 31 minutes and includes the images shown in Figure 2A-D.
Video 2
Movie 3 begins at T = 42 minutes and includes the images shown in Figure 2E-G. Frame-to-frame intervals were 5 seconds.
Video 3
Movies 4 and 5. Microtubule and endosome dynamics viewed by TIRF microscopy. Endosomes in cells expressing GFP-a-tubulin were labeled with the styryl dye FM 1-43; the cells were then treated with latrunculin-A. Movie 4 shows approximately 30 seconds of real-time activity in a labeled cell, revealing the continuous motion of microtubules and the intermittent association and transport of endosomes along them. The arrows point to moving endosomes. Note that the endosome marked in the first frame continues along a stretch of microtubule that is almost out of the narrow zone of illumination. The endosome reappears below, pauses, and then resumes movement. Presently that endosome (or another) makes a return trip along the same microtubule. Large, late endosomes tended to accumulate near the cell center, since their exocytosis was blocked by the presence of latrunculin-A.
Video 4
Movie 5 (first segment) shows the capture of an endosome by a moving microtubule. (See detailed description in legend to Figure 6B.) The sequence, which spans 2 seconds, is also shown in reverse. Movie 5 (second segment) illustrates how thoroughly the cytoplasm is explored by the coiling, undulatory motion of microtubules. This segment of the movie spans 20 seconds. All movies are shown at the same rate at which they were filmed (30 frames/second).
Video 5
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