Notch receptors are clustered and trans-endocytosed by Delta ligand cells. Confocal micrograph of a Delta expressing cell (left) interacting with a Notch expressing cell (right). Following interaction with Delta (blue), cell surface Notch (yellow) is clustered at cell-cell interfaces. Notch extracellular domain is detected within Delta cells (green) indicative of trans-endocytosis. Endocytosis of ligand while bound to Notch may produce a force sufficient to pull Notch apart and activate signaling.
 
 
 
 
 
 

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Volume 3 issue 11 November 2002, p. 833
The transitional ER defines a boundary for quality control in the secretion of tsO45 VSV glycoprotein
Anna Mezzacasa and Ari Helenius

Video 1, Dynamics of VSVG-GFP containing sites after heating up to 32°C as described in figure 5. Frames were taken every 5 s with an exposure time of 500ms, for a total of about 8 min. Playback: 15 frames per second.

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Video 1

Video 2 a, Same as movie 1, but the cells have been treated with 5µg/ml BFA 30 min prior and during the shift for 1 hour at 10°C. The drug was also present during in the medium during recording.

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Video 2a

Video 2 b, The same as movie 2a, but another cell.

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Video 2b

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