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Volume 4 issue 5 May 2003
Microtubule-dependent transport of secretory vesicles in RBL-2H3 cells
Alexander J. Smith*, Janet R. Pfeiffer*, Jun Zhang^, A. Marina Martinez*, Gillian M. Griffiths† and Bridget S. Wilson*
*Department of Pathology and Cancer Research & Treatment Center, University of New Mexico Health Sciences Center,
^ Department of Computer Sciences, University of New Mexico Albuquerque, New Mexico, 87107
†Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom, OX1 3RE
Video 1: Movement of GFP-FasL-labeled vesicles in a resting RBL-2H3 cell. Image sequence shows the movement of vesicles through a single confocal slice of the cell. Capture rate was 1 frame every 2 s; video is accelerated 24 fold.
Video 1
Video 2: Movement of GFP-FasL labeled vesicles in an activated RBL-2H3 cell. Image sequence shows the movement of vesicles in a single cell immediately before and after activation by crosslinking Fc R1 by addition of 100 ng/ml DNP-BSA. Capture rate was 1 frame every 4 s; video is accelerated 24 fold.
Video 2
Video 3: Fluorescence recovery after photobleaching in control cells. Resting control cells were subjected to photobleaching of the circled areas and the ability of granules to return to photobleached areas was monitored. Note that granules return rapidly to this area through linear movement along "tracks". Capture rate was 1 frame every 2s; video is accelerated 24 fold.
Video 3
Video 4: Fluorescence recovery after photobleaching in colchicine treated cells. Resting cells were pretreated with 100 M colchicine for 45 min. prior to imaging. The circled area was photobleached as in video 3. Note that granules are almost stationary and do not return to the photobleached area. Capture rate was 1 frame every 2s; video is accelerated 24 fold.
Video 4
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