Supp. Mat. Volume 4 Issue 7 July 2003 - Traffic the International Journal of Intracellular Transport

Notch receptors are clustered and trans-endocytosed by Delta ligand cells. Confocal micrograph of a Delta expressing cell (left) interacting with a Notch expressing cell (right). Following interaction with Delta (blue), cell surface Notch (yellow) is clustered at cell-cell interfaces. Notch extracellular domain is detected within Delta cells (green) indicative of trans-endocytosis. Endocytosis of ligand while bound to Notch may produce a force sufficient to pull Notch apart and activate signaling.

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Volume 4 issue 7 July 2003
Movement of plasma membrane associated clathrin spots along the microtubule cytoskeleton
Joshua Z. Rappoport, Bushra W. Taha and Sanford M. Simon

Video 1. TIR-FM video microscopy of region of MDCK cell transfected with dsRed-clathrin showing lateral motion of dsRed-clathrin spots. Acquisition time equals 300 ms per frame and each frame is played for 100 ms. The scale bar equals 2 mm.

Video 1

Video 2. TIR-FM video microscopy of region of MDCK cell co-transfected with dsRed-clathrin and tau-EGFP showing lateral motion of a dsRed-clathrin spot along a microtubule; Video 2 is the same region shown in Figure 2. Acquisition time equals 300 ms per frame and each frame is played for 100 ms.

Video 2