Volume 4 issue 9 September 2003

Video 1. EGF-stimulated translocation of wtPLC-1. wtPLC-ã1 located primarily in cytosol before EGF stimulation. After the addition of EGF, wtPLC-1 was translocated to plasma membrane within 5 min. Following EGF stimulation for 30 min, wtPLC-1 showed punctuate distribution in cytosol, suggesting internalization into endosomes.

Video 1WT

Video 2. EGF-stimulated translocation of SH3-PLC-1. Before EGF stimulation, SH3-PLC-1 was primarily cytosolic. After EGF stimulation for 5 min, strong membrane fluorescence was observed in cells transfected with SH3-PLC-1. After EGF stimulation for 30 min, a portion of SH3-PLC-1 was observed in endosomes.

Video 2SH

Video 3. EGF-stimulated translocation of NPH-PLC-1. NPH-PLC-1 was primarily cytosolic prior to EGF stimulation. After EGF stimulation for 5 min, strong membrane fluorescence was observed. Following EGF stimulation for 30 min, a small portion of NPH-PLC-1 was internalized into endosomes.

Video 3PH

Video 4. EGF-stimulated translocation of NSH2-PLC-1. Following EGF stimulation for 5 min, EGF-induced membrane localization of NSH2-PLC-1 was blocked. Even after stimulation for 30 min, no detectable fluorescence of PLC-1 was associated with the plasma membrane and endosomes in cells transfected with NSH2-PLC-1.

Video 4NS

Video 5. EGF-stimulated translocation of CSH2-PLC-1. Following EGF stimulation for 5 min, EGF-induced membrane localization of CSH2-PLC-1 was blocked. Even after stimulation for 30 min, no detectable fluorescence of PLC-1 was associated with the plasma membrane and endosomes in cells transfected with CSH2-PLC-1.

Video 5CS

Video 6. EGF-stimulated translocation of mPH-PLC-1. mPH-PLC-1 was primarily cytosolic prior to EGF stimulation. After EGF stimulation for 5 min, strong membrane fluorescence was observed. Following EGF stimulation for 30 min, a small portion of mPH-PLC-1 was internalized into endosomes.

Video 6PO