Supp. Mat. Volume 5 Issue 10 October 2004 - Traffic the International Journal of Intracellular Transport

Notch receptors are clustered and trans-endocytosed by Delta ligand cells. Confocal micrograph of a Delta expressing cell (left) interacting with a Notch expressing cell (right). Following interaction with Delta (blue), cell surface Notch (yellow) is clustered at cell-cell interfaces. Notch extracellular domain is detected within Delta cells (green) indicative of trans-endocytosis. Endocytosis of ligand while bound to Notch may produce a force sufficient to pull Notch apart and activate signaling.

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Volume 5 issue 10 October 2004
Temperature-sensitive random insulin granule diffusion is a prerequisite for recruiting granules for release.
Rosita Ivarsson, Stefanie Obermüller, Guy A. Rutter, Juris Galvanovskis and Erik Renström

Appendix 1: Laser scanning confocal imaging of a INS-1 cell showing that directed insulin granule movements occur along the microtubules. Microtubules were labelled with EGFP-tubulin and insulin granules with Lysotracker Red. Time interval between frames: 1,5 s.

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