Volume 5 issue 12 December 2004
3-D structure of multilaminar lysosomes in antigen presenting cells reveals trapping of MHC II on the internal membranes
Jean-Luc A.N. Murk¹*, Misjaël N. Lebbink²*, Bruno M. Humbel², Willie J.C. Geerts², Janice M. Griffith¹, Dennis M.L. Langenberg³, Frank A.W. Verreck³, Arie J. Verkleij², Abraham J. Koster², Hans J. Geuze¹, Monique J. Kleijmeer¹

1. Department of Cell Biology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands; 2. Department of Molecular Cell Biology, Institute of Biomembranes, Utrecht University, 3508 TC, Utrecht, The Netherlands and 3. Department of Immunohematology and Blood Transfusion, Leiden University Medical Center (LUMC), Albinusdreef 2, 2300 RC Leiden, The Netherlands
* Both authors contributed equally

Corresponding author:
Jean-Luc Murk
Department of Cell Biology
UMC Utrecht, AZU G02.525
Heidelberglaan 100, 3584 CX Utrecht
The Netherlands
E-mail: murk17@zonnet.nl / m.kleijmeer@quicknet.nl

Keywords: multilaminar lysosome, MHC II, electron tomography, antigen presentation, organelle biogenesis

Figure 2 MLL inner membranes have no connections with the outer membrane. Tomographic reconstructions in which the internal membranes could clearly be observed in the x-y plane, x-z and y-z plane (A) were manually traced (C). In each tomographic slice of about 4 nm, the membranes were drawn. When all drawn lines in the tomographic slices are combined, a 3-D model is generated (D and E). The example is taken from a MLL in a non-activated mouse DC

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