Supp. Mat. Volume 5 Issue 6 June 2004 - Traffic the International Journal of Intracellular Transport

Notch receptors are clustered and trans-endocytosed by Delta ligand cells. Confocal micrograph of a Delta expressing cell (left) interacting with a Notch expressing cell (right). Following interaction with Delta (blue), cell surface Notch (yellow) is clustered at cell-cell interfaces. Notch extracellular domain is detected within Delta cells (green) indicative of trans-endocytosis. Endocytosis of ligand while bound to Notch may produce a force sufficient to pull Notch apart and activate signaling.

Instructions to Authors

Supplemental Material

View our Privacy Policy

Supplemental Material

Volume 5 issue 6 June 2004
Requirement for the Rac GTPase in Chlamydia trachomatis Invasion of Non-phagocytic Cells
Rey A. Carabeo, Scott S. Grieshaber, Aaron Hasenkrug, Cheryl Dooley, and Ted Hackstadt

Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, NIAID, NIH, Rocky Mountain Laboratories, Hamilton , MT 59840

Running title: Chlamydial Requirement for Rac

Keywords: Chlamydia, Intracellular parasite, actin, GTPase, Rac

Corresponding author:
Ted Hackstadt, Ph.D.
Host-Parasite Interactions Section
Laboratory of Intracellular Parasites
NIAID, NIH
Rocky Mountain Laboratories
Hamilton, MT 59840
(406) 363-9308

Supplemental Movie 1: Chlamydia trachomatis recruits actin to the sites of binding and entry. Cos-7 cells expressing the actin-GFP (green) fusion protein were infected with CMTPX (red)-labeled C. trachomatis L2 EBs and observed using live cell confocal microscopy. The temperature throughout the experiment was maintained at 37 ° C using an objective heater. Time-lapse confocal images at a single focal plane were collected at 5 sec intervals.

View movie

Supplemental Figure 2: Chlamydia trachomatis recruits Rac to the sites of binding and entry. Cos-7 cells expressing the Rac-GFP (green) fusion protein were infected with CMTPX (red)-labeled C. trachomatis L2 EBs and observed using live cell confocal microscopy. The temperature throughout the experiment was maintained at 37 ° C using an objective heater. Time-lapse confocal images at a single focal plane were collected at 5 sec intervals.

View movie