Notch receptors are clustered and trans-endocytosed by Delta ligand cells. Confocal micrograph of a Delta expressing cell (left) interacting with a Notch expressing cell (right). Following interaction with Delta (blue), cell surface Notch (yellow) is clustered at cell-cell interfaces. Notch extracellular domain is detected within Delta cells (green) indicative of trans-endocytosis. Endocytosis of ligand while bound to Notch may produce a force sufficient to pull Notch apart and activate signaling.
 
 
 
 
 
 

Home

Aims and Scope

Editors

Contacts

Table of Contents

Article Search

Instructions to Authors

Submit an Article

Accepted Articles

Early View

Virtual Issues

Faculty of 1000

Supplemental Material

Cover Gallery

Subscribe

Etoc Alerts

Advertising

Links
 

 

  
Supplemental Material
 
 

Go back

Volume 6 issue 12 December 2005
Differential CD91 Dependence for Calreticulin and Pseudomonas Exotoxin-A Endocytosis
Julie Jo Walters and Brent Berwin

Supplementary Movies A, B and C: Endocytic trafficking of calreticulin, in conjunction with Pseudomonas Exotoxin-A, calreticulin, or IgG. Elicited peritoneal macrophages were incubated with Alexa647-labeled CRT (A, B, C) in combination with either Alexa488-labeled ExoA (A), Alexa488-labeled CRT (B), or Alexa488-labeled IgG (C) for 7 minutes at 37°C, followed by washing and fixation. CRT fluorescence is viewed as red in all movies, with ExoA, CRT and IgG viewed as green in Movies A, B and C respectively; co-localization is viewed as yellow. Microscopy was performed on a Zeiss LSM510 Meta microscope taking single optical sections with a 63x lens, which were reassembled as Z-series movies using LSM5 Image Browser.

Movie A (.avi 23.49MB)
Movie B (.avi 23.31MB)
Movie C (.avi 23.25MB)

Back to top