Volume 6 issue 12 December 2005
Differential CD91 Dependence for Calreticulin and Pseudomonas Exotoxin-A Endocytosis
Julie Jo Walters and Brent Berwin

Supplementary Movies A, B and C: Endocytic trafficking of calreticulin, in conjunction with Pseudomonas Exotoxin-A, calreticulin, or IgG. Elicited peritoneal macrophages were incubated with Alexa647-labeled CRT (A, B, C) in combination with either Alexa488-labeled ExoA (A), Alexa488-labeled CRT (B), or Alexa488-labeled IgG (C) for 7 minutes at 37°C, followed by washing and fixation. CRT fluorescence is viewed as red in all movies, with ExoA, CRT and IgG viewed as green in Movies A, B and C respectively; co-localization is viewed as yellow. Microscopy was performed on a Zeiss LSM510 Meta microscope taking single optical sections with a 63x lens, which were reassembled as Z-series movies using LSM5 Image Browser.

Movie A (.avi 23.49MB)
Movie B (.avi 23.31MB)
Movie C (.avi 23.25MB)